Amino acids sequences of KIC and CMI1 were underwent a multiple sequence alignment (MSA) using the MUSCLE algorithm ( ). The MSA results were converted into PIR format with the necessary adjustments to the solved crystal structure of KIC-KCBP complex (PDB ID code 3H4S) [67]. A pairwise alignment of KIC with CMI1 was extracted from the MSA PIR format and run using the Modeller 9.19 ( ). The built model of CMI1 was examined using the WHAT_CHECK (SAVES 5.0 server [ ]). The CMI1 model was visualized and edited using PyMOL ( ) and Adobe Photoshop CS6.
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